The study aimed to identify the attitudes of Jordanian university students towards digital culture in the light of the fourth industrial revolution. The descriptive approach was used, and the questionnaire was a tool for data collection. The study sample consisted of 550 students, as Yarmouk University was chosen to represent the northern region, and the University of Jordan to represent the central region, and Al-Hussein Bin Talal University to represent the southern region, and it was chosen in two ways: first, choosing a purposive sample to represent Jordanian universities, and then choosing a random sample. The results showed that there were differences in the arithmetic means according to the variables of gender and region. The study recommends developing comprehensive plans for the development of digital culture in Jordanian universities and including them in university courses to keep students abreast of developments.